Research Article

Antioxidant Effect of a Polyphenol-Rich Murtilla (Ugni molinae Turcz.) Extract and Its Effect on the Regulation of Metabolism in Refrigerated Boar Sperm

Figure 4

Analysis of metabolic parameters in sperm samples treated with MT-Ex. (a) Effect of MT-Ex on mitochondrial membrane potential. The data shown represent the cell count with normal potential measured by the JC-1 probe. (b) Succinate dehydrogenase activity evaluated by MTT assay. (c) Determination of ATP content. The groups were “control”—sperm samples incubated in the MR-A semen extender, “oxidant”—sperm samples treated with oxidant agent, “MT-Ex”—sperm samples treated with 0.0315 μgGAE mL-1 as antioxidant treatment, and “MT-Ex + oxidant”—sperm samples coincubated with oxidant agent and 0.315 μgGAE mL-1 as antioxidant treatment. Significant differences are represented between control and groups. , , and .
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