Dehydroepiandrosterone Prevents H₂O₂-Induced BRL-3A Cell Oxidative Damage through Activation of PI3K/Akt Pathways rather than MAPK Pathways

Figure 6

Effects of DHEA on PI3K, p-Akt, and MAPK protein expression in H₂O₂-treated BRL-3A cells. (a) Immunoblot of PI3K, p-Akt, and MAPKs in BRL-3A cells pretreated with 1-100 μM DHEA (24 h) and exposed to 150 μM H₂O₂ (2 h). (b) PI3K protein expression. (c) p-Akt protein expression. (d) p-p38 protein expression. (e) p-JNK protein expression. (f) p-ERK1/2 protein expression. (g) Immunoblot of PI3K and p-Akt in BRL-3A cells pretreated with 50 μM of the PI3K inhibitor LY294002 or vehicle for 1 h before exposure to 0, 1, 10, or 100 μM DHEA (24 h) and then 150 μM H₂O₂ (2 h). (h) PI3K protein expression. (i) p-Akt protein expression. Data are presented as the . compared to the vehicle-treated group; ^† and ^††compared to the group treated only with H₂O₂.

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