Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis
Table 1
Sequence of primers used in RT-qPCR.
Gene
Primer sequence 5-3
Product size
Melting temperature
Access number
β-actin
FW: CCCTGGCTCCTAGCACCAT
80 bp
60°C
NM_031144.3
RV: GATAGAGCCACCAATCCACACA
Nf-κb
FW: CCTCATCTTTCCCTCAGAGCC
98 bp
60°C
NM_199267.2
RV: CGCACTTGTAACGGAAACGC
Ki-67
FW: GGGTTTCCAGACACCAGACC
100 bp
60°C
NM_001271366.1
RV: CCAGGAAGACCAGTTAGAACC
Egf
FW: CTCAGGCCTCTGACTCCGAA
93 bp
60°C
NM_012842.1
RV: ATGCCGACGAGTCTGAGTTG
Vegf-A
FW: TGCGGATCAAACCTCACCAA
115 bp
60°C
NM_001110333.2
RV: GGCTCACAGTGATTTTCTGGC
Ho-1
FW: GTCCCAGGATTTGTCCGAGG
133 bp
60°C
NM_012580.2
RV: GGAGGCCATCACCAGCTTAAA
Ho-2
FW: CCGGGCAGAAAATACCCAGT
192 bp
60°C
NM_J05405.1
RV: ATCAGTGCTTCCTTCCGGTG
Gpx-1
FW: CATTGAGAATGTCGCGTCCC
141 bp
60°C
NM_030826.4
RV: TTGCCATTCTCCTGATGTCCG
Sod-2
FW: GTGGAGAACCCAAAGGAGAGT
177 bp
60°C
NM_017051.2
RV: GGTCCTGATTAGAGCAGGCG
Hif1-α
FW: ATCCATTTTCAGCTCAGGACAC
182 bp
60°C
NM_AF057308.1
RV: GGTAGGTTTCTGTAACTGGGTCTG
Nos-2
FW: GCCTAGTCAACTACAAGCCCC
87 bp
60°C
NM_012611.3
RV: AGAAACTTCCAGGGGCAAGC
Angpt-4
FW: AACTGTTCCAGAAGGTAGCCC
90 bp
60°C
NM_199115.2
RV: TCAAGAGGTCAATCTGGCTCTG
Col3α1
FW: GGGATCCAATGAGGGAGAAT
128 bp
60°C
NM_032085.1
RV: CCTTGCGTGTTTGATATT
FW: forward; RV: reverse; bp: base pairs. National Center for Biotechnology Information (NCBI) (Nucleotide, https://www.ncbi.nlm.nih.gov).
