Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 2

Research Article

SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway

Figure 2

SIRT7 regulated NO and ROS production in LPS-treated DCMECs. (a, b) DCMECs were transfected with three different SIRT7-targeted siRNAs. Knockdown efficiency was assessed by qRT-PCR and western blotting. (c) pcDNA3.0-SIRT7 was used for SIRT7 overexpression. Overexpression efficiency was assessed via western blotting. (d) NO was detected with DAF-FM DA. Scale bar: 100 μm; green: DAF-FM DA; blue: DNA. (e) ROS was labeled with DCFH-DA. Scale bar: 100 μm; green: DCFH-DA; blue: DNA. (f, g) Percentages of NO- and ROS-positive cells were calculated from the counts in five randomly selected visual fields. CTR cells are not transfected; NC (negative control) means the cells were transfected with nontargeting control siRNA; Ctrl (Control) means the cells were transfected with empty vector pcDNA3.0; SIRT7 cells were transfected with pcDNA 3.0-SIRT7. Data are expressed as and .

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