SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway

Figure 4

SIRT7 regulates nucleocytoplasmic translocation of NF-κBp-p65. (a, b) DCMECs were transfected with siSIRT7 or SIRT7 plasmid for 24 h and treated with LPS for 6 h. Immunofluorescence staining was conducted with an anti-NF-κBp-p65 antibody. Red: NF-κBp-p65; blue: DNA; scale bar: 50 μm. (c) The protein levels of NF-κBp-p65 in the nucleus and NF-κBp65 in the cytoplasm were analyzed via western blotting after the cells were transfected with siSIRT7 or SIRT7 plasmid.

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