Research Article
SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway
Figure 6
SIRT7 alleviates LPS-induced apoptosis in DCMECs. (a, b) DCMECs were transfected with siSIRT7 or pcDNA3.0-SIRT7 for 48 h and then treated with LPS for 6 h. The percentages of apoptotic cells were examined by flow cytometry. (c) The levels of Bax and Bcl-2 mRNA were determined via qRT-PCR. (d) The protein levels of Bax, Bcl-2, and cleaved-caspase-3 were determined by western blotting. Quantification of the relative protein levels are shown under the bands (protein/GAPDH). Data are expressed as .
(a) |
(b) |
(c) |
(d) |