Research Article
Hepatitis C Virus RNA-Dependent RNA Polymerase Is Regulated by Cysteine S-Glutathionylation
Figure 2
The recombinant NS5BΔ21 protein is subjected to S-glutathionylation which leads to reduction of RdRp activity. (a) A scheme showing impact of oxidized (GSSG) and reduced (GSH) glutathione on protein S-glutathionylation. (b) A western blot showing S-glutathionylation of the NS5BΔ21 protein. (c, d) RdRp activity of the NS5BΔ21 protein treated with glutathione isoforms or DTT, measured in primer-dependent (c) and primer-independent (d) assays. The results are presented as the . The control represents the untreated recombinant protein. and (Mann-Whitney test).
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