Research Article
Hepatitis C Virus RNA-Dependent RNA Polymerase Is Regulated by Cysteine S-Glutathionylation
Table 1
Cysteine residues of the recombinant NS5BΔ21 protein that are subjected to S-glutathionylation.
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123The peptide comprising aa 271-298 with three cysteines (C274, C279, and C295) contained two glutathione residues. In the same analysis, no glutathionylation was revealed in a peptide of aa 271-280. A peptide aa 223-259 with two cysteines (C223 and C243) contained one glutathione residue. In a similar sample, the peptide of aa 235-250 was not glutathionylated. The peptide comprising aa 271-298 contained two glutathione residues. In the same analysis, a glutathionylated peptide of aa 251-278 is implying that one of the residues is at C274, and the second is either at C279 or C295. |