Histone Acetyltransferase-Dependent Pathways Mediate Upregulation of NADPH Oxidase 5 in Human Macrophages under Inflammatory Conditions: A Potential Mechanism of Reactive Oxygen Species Overproduction in Atherosclerosis

Figure 3

IHC localization of p300, HAT1, H3K27ac, H3K9ac, and Nox5 proteins in the area of infiltrated immune cells/Mac, fibrous cap, and lipid-rich core of a human carotid atherosclerotic plaque. The images ((a) 4x magnification, (b) 10x magnification) show representative IHC staining of 5 μm thick serial sections from a human carotid atherosclerotic plaque for p300, HAT1, H3K27ac, H3K9ac, Nox5, CD45, CD68, and αSMA proteins. The red lesional lipid deposits were detected by Oil Red O staining. The positively stained cells are marked with arrows. Note the localization of Nox5 protein in the area of fibrous cap and lipid-rich core of atherosclerotic plaque expressing markers of infiltrated immune cells (CD45, CD68) and vascular SMCs (αSMA). The images are representative of 4 independent IHC assays.

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