FOXO1 Overexpression Attenuates Tubulointerstitial Fibrosis and Apoptosis in Diabetic Kidneys by Ameliorating Oxidative Injury via TXNIP-TRX

Figure 4

Effect of FOXO1 on apoptosis and interstitial fibrosis in the kidneys from diabetic mice. (a) Foxo1 mRNA levels in renal proximal tubules of normal, diabetic, and diabetic Tg mice detected by qPCR at week 12; Actb was used as an internal control. (b) Representative immunoblots. (c, d) Protein levels of the total FOXO1 and p-FOXO1 detected by western blot analysis at week 12; β-actin was used as an internal control. (e–g) Foxo1 overexpression prevented the increase in the expression of fibronectin (FN), collagen IV (Col IV), and BAX proteins. All data are expressed as , ( vs. the normal group and vs. the diabetes group).

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