H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS

Figure 1

Analysis of ROS intracellular amounts and FHC antioxidant protein levels in OVCAR3 and OVCAR8 cells. (a) Immunofluorescence analysis of ROS levels in OVCAR3 and OVCAR8 cells by staining with CellROX® Green Reagent (green). Nuclei were stained with DAPI (blue). Analysis was performed in duplicate and representative images are reported. (b) Immunofluorescence analysis of superoxide radical levels in OVCAR3 and OVCAR8 cells by staining with MitoSOX™ Red Indicator (red). Nuclei were stained with DAPI (blue). Analysis was performed in duplicate and representative images are reported. (c) Representative western blot of antioxidant protein FHC in OVCAR3 and OVCAR8 cells. γ-Tubulin was used as internal control. WB has been quantified by using ImageJ software and optical densitometry is reported. WB analysis was performed three times and results were reproducible.

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