H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS

Figure 2

OVCAR3 cells exhibit increased growth potential compared to OVCAR8 cells. (a) Cell cycle FACS analysis of OVCAR3 and OVCAR8 cells stained with PI. The experiments were performed in triplicate. Representative plots of a single experiment (left); histograms showing the of three independent experiments (right). value < 0.05, OVCAR3 vs. OVCAR8. (b) MTT analysis of OVCAR3 and OVCAR8 cell growth at 12 h, 24 h, 48 h, and 72 h. Data are reported as absorbance measured at 595 nm and shown as of three independent replicates (, OVCAR3 vs. OVCAR8); (°, OVCAR3 vs. OVCAR8); N.S.: not significant. (c) Representative WB of c-Myc, cyclin E1 (CCNE1), pChk2 (Thr68), pERK1/2, and pAKT in OVCAR3 and OVCAR8 cells. γ-Tubulin was used as internal control. WB analysis was performed three times and results were reproducible.

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