Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation

Figure 1

Expressions of Nrf2 and HO-1 in HEMA-exposed hDPCs. (a) Cytotoxicity of BHQ (25 μM) and ML385 (10 μM) after 24 h as evaluated by CCK-8 assay. No significant difference was observed among groups. (b) qRT-PCR analysis of mRNA expression levels of NFE2L2 and HMOX1 genes which encode Nrf2 and HO-1 proteins, respectively. (c) Western blot of Nrf2 and HO-1 protein expression levels. (d) Immunofluorescence analysis of Nrf2 expression and nuclear translocation. Green fluorescence represents Nrf2, and blue fluorescence represents nucleus of hDPCs. Data represent deviations (). , , and vs. untreated cells (control group); , , and vs. HEMA-treated cells. Data were analyzed using one-way analysis of variance (ANOVA) and post hoc Tukey test.

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