Research Article
Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation
| | Category | Gene | Forward | Reverse |
| | Nrf2 pathway | | | NFE2L2 | 5-CTTGGCCTCAGTGATTCTGAAGTG-3 | 5-CCTGAGATGGTGACAAGGGTTGTA-3 | | | HMOX1 | 5-CAGGAGCTGCTGACCCATGA-3 | 5-AGCAACTGTCGCCACCAGAA-3 | | Nuclear encoded ETC subunits | | NADH dehydrogenase (CI) | NDUFS8 | 5-CGTCGAGGGCCCCAACTTT-3 | 5-TAGTCAGCCTGGATGTTGGC-3 | | Succinate dehydrogenase (CII) | SDHB | 5-AAATGTGGCCCCATGGTATTG-3 | 5-AGAGCCACAGATGCCTTCTCTG-3 | | Cytochrome B (CIII) | UQCRC1 | 5-CAGTCCTCTCAGCCCACTTG-3 | 5-AAGCCAGATGCTCCAAAAAG-3 | | Cytochrome C oxidase (CIV) | COX5B | 5-GTTTTGGCTGCACAAAGGGC-3 | 5-CTGGGGCACCAGCTTGTAAT-3 | | ATP synthase (CV) | ATP5A | 5-AAGGAGATATAGTGAAGAGGACAG-3′ | 5-ATAAGTCGTCATAGATGATCAAAGC-3 | | mtDNA-encoded ETC subunits | | NADH dehydrogenase (CI) | ND1 | 5-CTCAACTTAGTATTATACCC-3 | 5-GGAAATACTTGATGGCAGCT-3 | | Cytochrome B (CII) | CYTB | 5TAGCAATAATCCCCATCCTCCATATAT-3 | 5-ACTTGTCCAATGATGGTAAAAGG-3 | | Cytochrome C oxidase (CIV) | COX1 | 5-ATTCGAGCAGAATTAGGTCA-3 | 5-CTCCGATTATTAGTGGGACA-3 | | ATP synthase (CV) | ATP6 | 5-TTTCCCCCTCTATTGATCCC-3 | 5-GTGGCCTTGGTATGTGCTTT-3 | | PGC1α/NRF1 pathway | | | PGC1α | 5-GTAAATCTGCGGGATGATGG-3 | 5-AATTGCTTGCGTCCACAAA-3 | | | NRF1 | 5-CTACTCGTGTGGGACAGCAA-3 | 5-AATTCCGTCGATGGTGAGAG-3 | | Control | GAPDH | 5-ATGACATCAAGAAGGTGGTG-3 | 5-CATACCAGGAATGAGCTTG-3 |
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