Auranofin Inhibits RANKL-Induced Osteoclastogenesis by Suppressing Inhibitors of κB Kinase and Inflammasome-Mediated Interleukin-1β Secretion

Figure 1

The inhibitory effect of auranofin on the resorption of CaP induced by RANKL. BMMs were isolated from C57BL/6N mice and cultured with 30 ng/mL M-CSF. After 3 days of incubation, adherent cells were incubated with 10% FBS/α-minimum essential medium containing 30 ng/mL M-CSF and 100 ng/mL RANKL and indicated the concentration of auranofin. For osteoclast formation, cells were stimulated with 100 ng/mL RANKL for 7 days. (a) Representative images of TRAP staining. (b) Number of TRAP-positive multinuclear cells (). (c) Representative images of pit formation assay on a CaP-coated plate. (d, e) The resorption activities were evaluated by fluorescence intensity and pit area. Data represents . compared with control group; and compared with the RANKL-treated group.

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