Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury

Figure 4

miR-219a-5p decreased the p66shc expression in ALD. (a) qRT-PCR analysis of the miRNA expression in different groups of rats (). and compared with ND; ^## and ^# compared with ALD. (b) qRT-PCR analysis of the miR-219a-5p expression in ethanol-exposed AML-12 cells (). and compared with the control group; ^## and ^# compared with the ethanol group. (c) The miR-219a-5p target sequence in the p66shc 3-UTR is conserved across various species. (d–g) AML-12 cells were transfected with ago-219a or ant-219a to upregulate or inhibit miR-219a-5p. Ago-NC or antago-NC was used as a negative control. (d, e) qRT-PCR analysis of miR-219a-5p expression (). (f, g) p66shc protein levels (). and compared with ago-NC or antago-NC.

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