Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury

Figure 6

PCA inhibited the p66shc expression via stimulation of miR-219a-5p. (a) Luciferase assay of AML-12 cotransfected with reporter constructs containing p66shc 3-UTR-wt or 3-UTR-mut and with ant-219a or antago-NC, as indicated (). (b) Schematic of the wild-type p66shc 3-UTR (3-UTR-wt) and mutated p66shc 3-UTR (3-UTR-mut) luciferase constructs. BS: binding site. (c, d) AML-12 cells were transfected with either ant-219a or antago-NC for 24 h and 10 μM protocatechuic acid treatment for 6 h. (c) qRT-PCR analysis of the miR-219a-5p expression (). (d) Protein levels of p66shc (). and compared with the antago-NC group; ^## and ^# compared with the ant-219a group.

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