Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury

Figure 7

PCA exerted an antioxidant effect through the miR-219a-5p/p66shc pathway with ethanol exposure. AML-12 cells were transfected with antogo-miR-219a-5p or antago-NC for 24 h, treated with 10 μM PCA for 6 h and/or 100 mM ethanol for 24 h. (a) qRT-PCR analysis of miR-219a-5p (). (b, c) Protein levels of p66shc and MnSOD (). (d) Hepatic GSH levels (), (e) hepatic MDA levels (), (f) hepatic CAT levels (), and (g) hepatic H₂O₂ levels (). (h) DCFH-DA staining. . and compared with the control group; ^## and ^# compared with the ethanol group; ^&& and ^& compared with cells transfected with ago-NC with ethanol.

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