Sulforaphane-Enriched Broccoli Sprouts Pretreated by Pulsed Electric Fields Reduces Neuroinflammation and Ameliorates Scopolamine-Induced Amnesia in Mouse Brain through Its Antioxidant Ability via Nrf2-HO-1 Activation

Figure 3

Pulsed electric field (PEF) and enzyme activity-treated broccoli sprouts inhibited expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in lipopolysaccharide- (LPS-) activated BV2 microglial cells. BV2 microglial cells were pretreated with broccoli extracts; LPS (100 ng/mL) stimulation was performed after 30 min. (a-c) iNOS and COX-2 expression and band intensity observed in LPS-activated BV2 microglial cells after a 6 h sample treatment and LPS activation. (d-f) iNOS and COX-2 expression and band intensity observed in a 24 h sample and LPS activation in BV2 cells. All data are presented as error of the mean of three independent experiments. , , and indicate significant differences compared with treatment with LPS alone, while indicates the significant differences compared with an untreated control group. Ctl: control; LPS: lipopolysaccharide; C: cotyledons; C-P: cotyledons exposed with PEF + enzyme activity; H: hypocotyls; H-P: hypocotyls exposed with PEF + enzyme activity.

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