Sulforaphane-Enriched Broccoli Sprouts Pretreated by Pulsed Electric Fields Reduces Neuroinflammation and Ameliorates Scopolamine-Induced Amnesia in Mouse Brain through Its Antioxidant Ability via Nrf2-HO-1 Activation

Figure 6

Pulsed electric field (PEF) and enzyme activity-treated broccoli sprouts inhibited NF-κB translocation and I-κB phosphorylation in lipopolysaccharide- (LPS-) activated BV2 cells. BV2 microglial cells were pretreated with broccoli extracts; LPS (100 ng/mL) stimulation was performed after 30 min. NF-κB, I-κB, and pI-κB expression was observed after 1 h of LPS activation. (a, b) Nuclear NF-κB expression and band intensity. Histone-3 was used as loading control. (c, d) Cytosolic NF-κB expression and band intensity. β-Actin was used as loading control. (e, f) Cytosolic I-κB and pI-κB expression and band intensity. β-Actin was used as loading control. All data are presented as error of the mean of three independent experiments. , , and indicate significant differences compared with treatment with LPS alone, while , , and indicate significant differences compared with an untreated control group. Ctl: control; LPS: lipopolysaccharide; C: cotyledons; C-P: cotyledons exposed with PEF + enzyme activity; H: hypocotyls; H-P: hypocotyls exposed with PEF + enzyme activity.

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