Inhibition of Mitofusin-2 Promotes Cardiac Fibroblast Activation via the PERK/ATF4 Pathway and Reactive Oxygen Species

Figure 5

Mfn2 regulated the generation of ROS in activated cardiac fibroblasts. (a) The ROS level in cardiac fibroblasts treated with TGF-β1 for 24 h; (b) the ROS level in cardiac fibroblasts after overexpression adenovirus transduction; adenovirus transduction in the presence or absence of TGF-β1; (c) the ROS level in cardiac fibroblasts after siMfn2 transfection; siMfn2 transfection in the presence or absence of TGF-β1; (d) the protein levels of α-SMA, TGF-β, and CTGF in cardiac fibroblasts after transfection with siMfn2 (); siMfn2 transfection in the presence or absence of NAC. Data in (a–d) are expressed as . NS: no significance. indicates , indicates , and indicates vs. the NC groups.

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