Bloom Syndrome Protein Activates AKT and PRAS40 in Prostate Cancer Cells

Figure 8

BLM, pAKT, and pPRAS40 expression in LNCAP cells. (a) BLM helicase, pAKT, and pPRAS40 expression in LNCAP cells as measured by western blot and WES analysis. (b) Cells treated with BEZ235 and GDC-0068 displayed significantly reduced BLM expression. BLM expression values were normalized to β-actin, and pAKT and pPRAS40 (Thr246) expression values were normalized to total AKT1 and PRAS40, respectively. Data represent the mean ± SD ( and ). BLM expression is positively correlated with (c) pAKT () and (d) pPRAS40 levels ().

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