Nrg1 Intracellular Signaling Is Neuroprotective upon Stroke

Figure 3

Nrg1 intracellular signaling does not affect the activation of p38 MAPK and DNA damage in vitro. (a–d) Representative images of neurons subjected to in vitro aging expressing either GFP as control or Nrg1-ICD labeled by GFP (a, b) and phospho-p38 MAPK (c, d). The color bar on the right illustrates the lookup table (LUT) for signal intensity. Scale bar, 20 μm. (e) Graph illustrates the quantification of phospho-p38 MAPK labeling expressed in arbitrary units (a.u.). fields from two neuronal cultures. , -test. . (f–i) Representative images of aged primary neurons treated as in (a–d). Labeling shows GFP (f, g) and phospho-H2AX (h, i). Scale bar, 20 μm. (j) The graph shows the percentage of GFP or Nrg1-ICD-expressing neurons positive for phospho-H2AX. fields from two neuronal cultures. , -test. .