Nrg1 Intracellular Signaling Is Neuroprotective upon Stroke

Figure 5

Nrg1 intracellular signaling inhibits neuronal loss in an in vitro model of stroke. (a) Representative images of neurons labeled for GFP and MAP2. The neurons were infected to express either GFP as control or Nrg1-ICD or Nrg1-FL (both Nrg1 constructs are tagged with GFP). OGD was performed at D14, and the neurons were fixed 24 hours after OGD. Arrowheads indicate the surviving neurons. Scale bar, 50 μm. (b) The graph illustrates the quantification of neuronal survival upon OGD compared to control conditions. fields out of three cultures. , , two-way ANOVA, and Dunnett’s multiple comparisons test. .

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