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Figure 3: Melatonin alleviated mitochondrial oxidative stress in vitro and in vivo. (a) The correlation between different concentrations of H2O2 and ROS production ( per group, and vs. the control group). (b) The correlation among different concentrations of melatonin (0, 25, 50, 100, and 200 μM) and cell viability without H2O2, per group. (c) The correlation among different concentrations of melatonin (0, 25, 50, 100, and 200 μM) and cell viability with H2O2 (400 μM) ( per group, and vs. the control group, # and ## vs. the H2O2 group). (d) Melatonin (100 μM) alleviated oxidative stress in the H2O2 (400 μM) model ( per group, and vs. the control group, # and ## vs. the H2O2 group). (e–i) Western blot of cyto-c, NOX2, NOQ1, and Nrf2 ( per group, and vs. the control group, and vs. the H2O2 group). (j–l) Immunostaining of cyto-c and NOX2 ( per group, and ).