Melatonin Increases Bone Mass around the Prostheses of OVX Rats by Ameliorating Mitochondrial Oxidative Stress via the SIRT3/SOD2 Signaling Pathway

Figure 3

Melatonin alleviated mitochondrial oxidative stress in vitro and in vivo. (a) The correlation between different concentrations of H₂O₂ and ROS production ( per group, and vs. the control group). (b) The correlation among different concentrations of melatonin (0, 25, 50, 100, and 200 μM) and cell viability without H₂O₂, per group. (c) The correlation among different concentrations of melatonin (0, 25, 50, 100, and 200 μM) and cell viability with H₂O₂ (400 μM) ( per group, and vs. the control group, ^# and ^## vs. the H₂O₂ group). (d) Melatonin (100 μM) alleviated oxidative stress in the H₂O₂ (400 μM) model ( per group, and vs. the control group, ^# and ^## vs. the H₂O₂ group). (e–i) Western blot of cyto-c, NOX2, NOQ1, and Nrf2 ( per group, and vs. the control group, and vs. the H₂O₂ group). (j–l) Immunostaining of cyto-c and NOX2 ( per group, and ).

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