Figure 5: Activation of the SIRT3/SOD2 signaling pathway was essential to the antioxidative effects of melatonin. (a, b) The efficiency of SIRT3 siRNA was confirmed by Western blot ( per group, && vs. the scramble SiRNA group). (c–h) Western blot of SIRT3, Ac-SOD2, SOD2, cyto-c, NOQ1, and Nrf2 ( per group, and vs. the H2O2 group, # and ## vs. the H2O2 + MT group).