Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 4

Research Article

Upregulation of Transient Receptor Potential Canonical Type 3 Channel via AT1R/TGF-β1/Smad2/3 Induces Atrial Fibrosis in Aging and Spontaneously Hypertensive Rats

Figure 4

TRPC3 inhibition prevents Ang II-induced cell migration and proliferation. (a). After wound healing assay, the wound of the 24-well cultured confluent neonatal rat atrial fibroblasts was imaged before and after administration of Pyr3 (10 μmol/L) and Ang II with or without Pyr3 (10 μmol/L) for 48 h; the wound edges were imaged under a 10x objective. (b) Accurately measure cell migration by transwell assay. Transwells are placed in wells, and cell suspension is pipetted into the upper chambers, incubating the cells with administration of Pyr3 (10 μmol/L) and Ang II with or without Pyr3 (10 μmol/L) for 48 h; image the migrated fibroblasts from underside membrane, count the migrated cells on a grid under magnification ×200, and count 5 high power fields to calculate the average cell number per field. All data shown are the and are expressed as % of the control value (first bar). versus control (the first ) and versus Ang II (the second ).
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