Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway
Effect of ZER on cell viability and altered MMP-1 and collagen III expressions in UVA-irradiated HSF cells. (a) Chemical structure of zerumbone (ZER). (b) Cells were pretreated with ZER (0, 2, 4, or 8 μM for 24 h) followed by UVA irradiation (3 J/cm2 for 27 min). 24 h after UVA exposure, the percentage of cell viability was measured by MTT colorimetric assay as described. The formula used to calculate the percentage of viable cells was . (c, d) HSF cells were pretreated with ZER (0, 2, 4, or 8 μM for 24 h), and the expression of MMP-1 and collagen III proteins in the absence (c) or presence (d) of UVA radiation was measured using the western blot method. Results were presented as of three or more assays. ,, compared to untreated control cells; #,## compared to UVA-irradiated cells.