Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway
Effect of ZER on intracellular ROS levels and SA-β-gal activity in UVA-irradiated HSF cells, (a) Cells were pretreated with ZER (0, 2, 4, or 8 μM for 24 h) followed by irradiated with UVA in the absence or presence of 3 J/cm2 UVA. The accumulation of UVA-induced ROS and SA-β-gal-positive cells was measured using fluorescence microscopy (200x magnification) as described. (b) The data was represented as fold change over control intracellular ROS levels. (c) The data was reported as fold increase over control SA-β-gal-positive cells. Results from three or more experiments were presented as , and the statistical significance was considered as compared to untreated control cells; #,##,### compared to UVA-irradiated cells.