Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson’s Disease Model

Figure 1

CP protects against neuronal cell loss induced by PQ in rat primary dopaminergic neurons and the SN4741 dopaminergic neuronal cell line. (a, b) Primary dopaminergic neurons isolated from the ventromedial area of the mesencephalic region of E14 rats were pretreated with 1 μg/ml CP for 24 h and then treated with 0–16 μM PQ for 24 h. Dopaminergic neurons were identified by immunofluorescence staining with an anti-TH antibody. (a) Immunofluorescence-stained TH+ cells were confirmed by fluorescence microscopy TH+ cells, green; nuclei, blue. Scale bars, 100 μm. (b) Bar graph showing quantification of TH+ cells, confirmed by fluorescence microscopy (). (c) SN4741 cells were pretreated with 2.5 or 10 μg/ml CP for 24 h. Cells were further treated with 0–800 μM PQ for 24 h, and the survival rate of dopaminergic neurons was confirmed using CCK8 (). (d) Bar graph showing cell viability (). All data are representative of three independent experiments. by 2-tailed unpaired Student’s -test in (b) and by one-way ANOVA in (c, d). Error bars represent +SD.

(a)

(b)

(c)

(d)