Protective Effect of Ethyl Pyruvate against Myocardial Ischemia Reperfusion Injury through Regulations of ROS-Related NLRP3 Inflammasome Activation

Figure 6

EP reduced hypoxia-induced TXNIP-NLRP3 expressions and their interaction partly via ERK and p38 pathway in H9c2 cardiomyocytes. Immunoprecipitation (IP) and immunoblot (IB) assays of TXNIP and NLRP3 expressions, and their interaction in normoxic (N) or hypoxic (H) conditions in serum-starved H9c2 cardiomyocytes (a). Immunoblot assays of P-ERK, ERK, P-p38, and p38 expressions in a hypoxic condition in serum-starved H9c2 cardiomyocytes treated either with or without EP for 24 h (b). Serum-starved H9c2 cells were transiently transfected with a Myc-TXNIP and GFP-NLRP3 expression plasmids, incubated in the presence or absence of EP for 24 h under hypoxia and subjected to IP and IB analysis (c). EP, ethyl pyruvate; TXNIP, thioredoxin-interacting protein; NLRP3, nucleotide-binding oligomerization domain-like receptor with a pyrin domain 3; U0126, MEK inhibitor; SB203580, p38 inhibitor.

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