Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis

Figure 1

H₂O₂ increased Cul4a and apoptosis-related protein and decreased the viability of H9c2 cardiomyocytes. (a) H9c2 cells were untreated or treated with 50 μM, 100 μM, 200 μM, and 400 μM H₂O₂ for 2 h. Cell viability was assessed by CCK-8 assay. (b) Flow cytometry Annexin-FITC/PI showed that the apoptosis rate of cell increased gradually induced by gradient concentration of H₂O₂. (c) Immunofluorescence staining of H9c2 with Cul4a antibody (green) and DAPI for nuclei (blue) after treatment with 200 μM of H₂O₂ for 2 h. (d) Western blot analysis of Cul4a, cleaved PARP1 and cleaved caspase3 expression after incubation with H₂O₂ (50 μM, 100 μM, 200 μM, and 400 μM) for 2 h; α-tubulin was used as a loading control. , , ; scale bar, 50 μm. Data was expressed as .

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