Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis

<div>Cul4a overexpression inhibited H<sub>2</sub>O<sub>2</sub>-induced cardiomyocyte apoptosis. (a) H9c2 cells were untreated or treated with 50 <i>μ</i>M, 100 <i>μ</i>M, 200 <i>μ</i>M, and 400 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> for 2 h. CCK-8 assay results showed that overexpressed Cul4a decreased H<sub>2</sub>O<sub>2</sub>-induced cell cytotoxicity. (b) Flow cytometry Annexin-FITC/PI showed that overexpressed Cul4a reduced H<sub>2</sub>O<sub>2</sub>-induced cell apoptosis. (c) Overexpressed Cul4a significantly decreased H<sub>2</sub>O<sub>2</sub>-induced apoptotic marker proteins cleaved PARP1 and cleaved caspase-3 expression by western blot assay; <i>α</i>-tubulin was used as a loading control. <span class="nowrap"><svg height="13.532pt" id="M8" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 68.0304 13.532" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 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374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z"></path></g><g transform="matrix(.013,0,0,-0.013,53.139,0)"><path d="M43 650V622C120 616 128 612 128 526V124C128 39 120 33 34 27V0H270C392 0 492 25 567 83C643 141 690 230 690 350C690 444 655 517 605 565C543 625 450 650 323 650H43ZM213 547C213 587 217 598 226 604C236 612 262 617 304 617C371 617 429 604 474 576C554 529 592 439 592 336C592 176 505 36 319 36C246 36 213 55 213 131V547Z"></path></g></svg>.</span> Cul4a: cullin 4a; PARP1: poly (ADP-ribose) polymerase-1.</div>

Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis 