Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs

Table 2

LC/MS data and assignment of 15 bioactive constituents in PR extract.

No.	(min)	∆P^b (%, )	UV (nm)	[M+H]^+ ([M+Na]^+)	ESI-MS^n(+) (abundance)	[M-H]^-	ESI-MS^n(-) (abundance)	Predicted formula	Meas. ()	Pred. ()	Diff. (ppm)	DBE	Assigned identification
P1	31.593		200	409.1899	MS^2 (409):247 (100)	407.0915	MS^2 (407):361 (100), 362 (23), 354 (16), 283 (16), 248 (15), 108 (15), 230 (14), 219 (14), 202 (14), 154 (12), 104 (11)	C23H36O6	408.1502	408.2597	-1.568	6	Unidentified
P2	44.173		220, 255	367.1151	MS^2 (367):209 (100), 210 (10)	365.2104	MS^2 (365):329 (100), 191 (10), 298 (10)	C18H6O9	366.2184	366.0030	-1.73	16	(−)-cis-(3R,4R)-3-angeloylkhellactone or (−)-cis-(3R,4R)-4-angeloylkhellactone
P3	49.950		325	367.1154	MS^2 (367):267 (100), 203 (16); MS^2 (345):203 (100), 204 (14), 187 (10); MS^3 (203):203 (100), 175 (27), 204 (15)	343.9714	MS^2 (343):99 (100), 261 (91), 203 (75), 175 (32), 262 (25), 280 (15), 243 (12), 229 (11); MS^3 (261):203 (100), 175 (76), 69 (64), 145 (53), 189 (42), 223 (22), 161 (21), 119 (21), 89 (17), 195 (11), 118 (11), 186 (11)	C12H8O12	344.1154	344.0040	-2.99	9	Pd-C-I
P4	51.160		200, 325	367.1143	MS^2 (367):267 (100), 203 (23), 204 (15), 268 (14), 367 (12)	365.2106	—	C18H6O9	366.2145	366.0030	-1.73	16	(−)-cis-(3R,4R)-3-angeloylkhellactone or (−)-cis-(3R,4R)-4-angeloylkhellactone
P5	53.397		325	367.1150	MS^2 (367):267 (15)	343.1196	MS^2 (343):215 (100), 99 (60), 95 (16), 69 (12), 172 (10), 151 (10)	C12H8O12	344.1270	344.0040	-2.99	9	(+)-trans-(3S, 4R)-3-angeloylkhellactone
P6	58.633		200, 325	376.1389	MS^2 (376):245 (100), 227 (50), 287 (17), 246 (14); MS^2 (245):203 (84), 175 (24), 145 (20), 246 (15), 204 (13)	351.2317	MS^2 (351):59 (100), 277 (75), 351 (75), 352 (16), 278 (13)	C17H20O8	352.2133	352.1200	13.44	8	Unidentified
P7	60.897		200, 255, 320	369.0947	MS^2 (369):267 (100), 369 (30), 203 (22), 309 (19), 227 (10); MS^3 (267):143 (100), 102 (82), 161 (60), 184 (53), 267 (43), 269 (36), 145 (21), 238 (13), 91 (10)	—	MS^2 (345):299 (100), 277 (65), 300 (22), 69 (21), 95 (16), 278 (14)	C18H18O7	346.1107	346.0947	15.30	10	Qianhucoumarin D
^aP8	63.003		200, 325	409.1261	MS^2 (409):245 (100), 227 (41); MS^3 (245):189 (100), 144 (77), 180 (32), 175 (32), 163 (30), 187 (24), 161 (24), 186 (20), 83 (17), 198 (11)	385.3148	—	C21H22O7	386.3178	386.1410	10.35	11	Praeruptorin A
P9	63.300		200, 325	409.1261	MS^2 (409):245 (100), 309 (93), 227 (35), 409 (30), 83 (25), 310 (21), 246 (17); MS^3 (245):175 (48), 189 (16), 246 (16), 217 (15), 187 (14)	385.3148	—	C21H22O7	386.3178	386.1410	10.35	11	Unidentified
P10	68.660		200, 325	411.1402	MS^2 (411):217 (100), 175 (83), 187 (71), 92 (56), 201 (51), 83 (42), 69 (42), 53 (36)	387.1149	—	C21H24O7	388.1180	388.1570	-4.26	10	Peucedanocoumarin I
P11	69.150		205	449.1563	MS^2 (449):245 (100), 227 (71), 246 (17), 175 (14), 228 (13)	425.3047	—	C22H18O9	426.2538	426.0990	12.83	14	Unidentified
^aP12	69.713		200	449.1562	MS^2 (449):83 (100), 227 (21), 349 (13)	425.3046		C24H26O7	426.2535	426.0990	12.83	14	Praeruptorin B
^aP13	70.043		200	449.1658	MS^2 (449):83 (100), 227 (30), 55 (17), 349 (17), 327 (13)	425.3046	—	C24H26O7	426.2538	426.0990	12.83	14	Praeruptorin D
P14	71.390		200	449.1572	MS^2 (449):409 (100)	425.3050		C24H26O7	426.2535	426.0990	12.83	14	Unidentified
^aP15	71.927		200	451.1780	MS^2 (451):227 (37), 327 (17), 349 (15)	427.2556	—	C24H28O7	428.3917	428.1890	-6.90	11	Praeruptorin E

^aCompared with standards. ^b was calculated using the following equation: , where and are the peak areas in the experiment and control, respectively. Data were obtained from 3 independent experiments and are expressed as .