Hypoxia-Inducible Factor-1α Knockdown Plus Glutamine Supplementation Attenuates the Predominance of Necrosis over Apoptosis by Relieving Cellular Energy Stress in Acute Pancreatitis

Figure 1

The upregulation of HIF-1α after AP induction was prevented by prior HIF-1α knockdown. (a) Representative EMSA blots (left) and quantifications (right) of HIF-1α DNA-biding activity in pancreatic tissues harvested from the rats that were subjected to sham operation, AP, HIF-1α siRNA, and HIF-1α siRNA+Gln for 24 h since AP induction. (b) Representative immunoblot images (left) and quantifications (right) of HIF-1α protein expression in pancreatic tissues harvested from the rats as described above. β-Actin was used as the protein loading control. Data were presented as (). versus sham, ^{^}versus AP, and ^▽versus HIF-1α siRNA. Abbreviations: AP: acute pancreatitis; EMSA: electrophoretic mobility shift assay; Gln: glutamine; HIF-1α: hypoxia inducible factor-1α; SD: standard deviation.

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