Hypoxia-Inducible Factor-1α Knockdown Plus Glutamine Supplementation Attenuates the Predominance of Necrosis over Apoptosis by Relieving Cellular Energy Stress in Acute Pancreatitis

Figure 8

HIF-1α knockdown decreased necrosis and increased apoptosis within AR42J cells after AP induction, and these effects were enhanced when Gln was administered concomitantly with HIF-1α knockdown. (a and c) Representative fluorescent images (a, 10x) indicative of apoptotic activity by Annexin V and propidium iodide double-staining in AR42J cells as described in Figure 6(a), and the percentage of green cells (c) was calculated to assess the apoptotic cells rate. (b and d-f) Representative immunoblot images (b) and quantifications of cytoplasm cleaved caspase-3 (d), caspase-9 (e), and PARP (f) expression in AR42J cells as described in Figure 6(a). β-Actin was used as the protein loading control. Data were presented as (). versus sham, ^{^}versus AP, and ^▽versus HIF-1α siRNA. Abbreviations: AP: acute pancreatitis; Gln: glutamine; HIF-1α: hypoxia-inducible factor-1α; SD: standard deviation.

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