Antioxidant Properties of Fullerene Derivatives Depend on Their Chemical Structure: A Study of Two Fullerene Derivatives on HELFs

Figure 8

DNA damage in cells incubated with GI-761 and VI-419-P3K. (a–c) Comet assay: (a) digital photography of nuclei with varying degree of DNA damage; (b) dependence of “% of tail DNA” on fullerene derivative concentration; (c) dependence of DNA comet tail moment on the time of incubation with fullerene derivatives. (d) Fluorescent microscopy: cells stained with antibodies for H2AX. (e) FCA: average signal intensity of FL1-γH2AX given for times and concentrations of investigated fullerene derivatives.

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