Cucurbitacin B Exerts Antiaging Effects in Yeast by Regulating Autophagy and Oxidative Stress
CuB neither inhibits cell growth nor causes morphological changes. (a) The growth curve of Saccharomyces cerevisiae in the presence and absence of CuB was plotted. The assay was repeated at least twice. (b) Images of cells treated with control (1% EtOH) or CuB (10 μM). The fluorescent dyes fluorescein isothiocyanate-Con A, 4,6-diamidino-2-phenylindole, and rhodamine-phalloidin were used to stain the cell wall, nuclear DNA, and actin, respectively. Scale bar, 5 μm. (c) Scatter plot of mutants with nonessential gene deletions in terms of specific morphological abnormality (-axis) and growth rate (-axis). The horizontal and vertical red lines indicate the false discovery rate of 0.01 for growth rate and morphology, respectively. The number shown in each area indicates the number of mutants categorized on the basis of cell growth and morphology (single-gene deletion strains with increased lifespan/mutants categorized on the basis of cell growth and/or morphology).
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