ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt c/Caspase-3 Pathway

<div>Mitochondrial dysfunctions caused by mtKR-induced ROS and X-rays. (a) The changes of Na<sup>+</sup>-K<sup>+</sup> and Ca<sup>2+</sup>-Mg<sup>2+</sup> ATPase activities and ATP content by biochemical assay after light exposure and irradiation. (b) The FCM pictures of Δ<i>ψ</i><sub>m</sub>. HeLa cells were stained by Rh123, followed by FCM analysis of the Δ<i>ψ</i><sub>m</sub>. (c) Western blot analysis was performed to determine the protein levels of VDAC1 in total and mitochondrial protein. GAPDH and HSP60 proteins were used for loading control. (d) From top to bottom, the gray ratios of VDAC1/GAPDH and VDAC1/HSP60. 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604C236 612 262 617 304 617C371 617 429 604 474 576C554 529 592 439 592 336C592 176 505 36 319 36C246 36 213 55 213 131V547Z"></path></g></svg> of triplicate measurements. <svg height="10.7395pt" id="M27" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt c/Caspase-3 Pathway 