ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt c/Caspase-3 Pathway

Figure 5

Apoptosis induced by mtKR and irradiation via Cyt c/caspase-3 pathway. (a) The FCM pictures of apoptosis and FCM analysis in HeLa cells stained by Annexin V/FITC and PI; the apoptotic population was defined as early apoptosis (lower right, green of FITC staining). (b) Cyt c, caspase-9, and caspase-3 mRNAs were detected by qRT-PCR. (c) Western blot was performed to determine the protein levels of Cyt c, caspase-9, and caspase-3 in total and mitochondrial proteins. GAPDH and HSP60 proteins were used for loading control. (d) From top to bottom, the gray ratios of cleaved caspase-9/GAPDH, cleaved caspase-3/GAPDH, Cyt c/GAPDH, and Cyt c/HSP60. The bars represent the of triplicate measurements. , , and versus control; versus 4 Gy irradiation, and versus light exposure.

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