Research Article

Adaptations in Protein Expression and Regulated Activity of Pyruvate Dehydrogenase Multienzyme Complex in Human Systolic Heart Failure

Figure 2

(a) Relative PDH activity in nonfailing () and heart failure () groups normalized to citrate synthase ( vs nonfailing). Citrate synthase protein expression did not differ between groups. (b) Example of PDH subunits and inner mitochondrial membrane CV detected concurrently by Western blot. CV = complex V, ATP synthase F1α subunit. (c) Mean (±SD) protein expression of PDH subunits E2, E3bp, E1a, and E1b, determined by Western blotting ( vs nonfailing).