Adaptations in Protein Expression and Regulated Activity of Pyruvate Dehydrogenase Multienzyme Complex in Human Systolic Heart Failure
Summary of mitochondrial pyruvate carrier (MPC) and pyruvate dehydrogenase phosphatase (PDP) protein expression determined by Western immunoblot assays. (a) Relative MPC1 and MPC2 protein expression in nonfailing () and heart failure (), normalized to porin ( vs nonfailing). (b) Western immunoblot band examples from independent MPC1 and MPC2 assays (nonfailing and heart failure groups measured concurrently). (c) Relative PDP1 and PDP2 protein expression (normalized to porin) in nonfailing () and heart failure ().