Reduced Liver Lipid Peroxidation in Subcellular Fractions Is Associated with a Hypometabolic State in Rats with Portacaval Anastomosis

Table 1

Morphometric and biochemical parameters in rats with PCA.

	Sham		PCA
	Mean	SEM	Mean	SEM
Body weight (g)	444.0	15.0	335.6	16.0
Liver weight (g)	15.5	0.7	7.3	0.4
Liver dry weight (%)	71.7	0.8	72.4	1.1
Blood
Ammonium (μg/ml)	2.1	0.2	4.0	0.4
Urea (mg/dl)	56.8	2.9	51.2	1.6
Glucose (mg/dl)	135.4	7.0	113.3	3.2
TAG (mg/dl)	46.4	6.6	44.5	4.2
ALT (U/l)	10.6	1.1	17.7	3.0
AST (U/l)	282.5	13.6	191.8	2.9
Liver
Glutamine synthetase (cytosol) (μmoles NADH/min/mg)	10.9	1.4	6.6	1.2
Glutamate dehydrogenase (mitochondria) (μmoles NADH/min/mg)	317.6	40.6	190.5	12.6
Caspase 3 (absorbance at 405 nm)	1.5	0.3	2.2	0.2

Body and liver weight, blood parameters, and liver enzymatic activities were determined in rats with PCA 13 weeks after surgery. Blood determinations were performed in the serum and liver determinations were done in homogenate and subcellular fractions. TAG: triacylglycerols; ALT: alanine aminotransferase; ASP: aspartate aminotransferase. Significant differences were detected in cytoplasmic glutamine synthetase activity and in mitochondrial glutamate dehydrogenase activity. No changes were detected in the activity of both enzymes in the liver homogenate. Values correspond to 7-12 sham rats and 15-19 PCA rats. Statistical significance () was calculated by the Student -test; value was set at 0.05.