Folic Acid Supplementation Suppresses Sleep Deprivation-Induced Telomere Dysfunction and Senescence-Associated Secretory Phenotype (SASP)

Figure 1

Folic acid attenuates sleep deprivation-induced oxidative stress and SASP disorder. (a) Experimental design. A schematic diagram showing that mice were fed a long-term folic acid-supplemented diet for more than two months, and sleep deprivation was performed for 20 h/day (01:00 pm to 09:00 am the following day) for 7 days. (b) The folic acid concentration in serum before and after folic acid deficiency (or supplementation). (c) Reactive oxygen species (ROS) in the liver was assayed using DCFH-DA as a probe. (d) Antioxidative capacity in the liver was detected by the total SOD assay kit with WST-8. (e, f) Secretion of tumor necrosis factor-α (TNF-α) in the plasma, detected by ELISA, and the fold change (SD/WC) in the FAD or FAS groups. (g) Secretion of interleukin-6 (IL-6) in the plasma, detected by ELISA. (h–k) The abundance of p53 and p16^INK4a in the plasma was detected by ELISA and the fold change (SD/WC) in the FAD or FAS groups. Data are presented as the ( per group). ; ; and .

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