NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway

Figure 8

The effects of Nox inhibition on oxidant stress damage and cell apoptosis in mimic SAP-induced cardiac injury in vitro. The CCK-8 assay shows that 10% SAP rat serum was the appropriate concentration and 12 h was the suitable time (a). Nox inhibition attenuates ROS production and apoptosis of H9C2 cardiomyocyte stimulated by SAP serum in vitro. Intracellular ROS level was detected by using DHE staining. The fluorescence values are expressed as the ratio to the levels in the NS group cells (b and c). Flow cytometry analysis of apoptosis in H9C2 cardiomyocytes by using Annexin V FITC/PI double staining (d). NS, normal rat serum group; SS, SAP rat serum group; SA, SAP rat serum and apocynin group; NA, normal rat serum and apocynin group. compared with the NS group. compared with the SS group.

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