MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI

Figure 2

AMO-155 attenuated cardiomyocyte apoptosis in response to H₂O₂. NRVMs were transfected with NC, miR-155, AMO-155, and miR-155+AMO-155 (155+AMO) and then treated with H₂O₂ (100 μM) for 4 h. (a) Representative images of TUNEL staining of NRVMs for DNA defragmentation showing the apoptotic cells (nucleus stained in blue with DAPI and apoptotic cells stained in green). (b) Quantification analysis of total TUNEL-positive cells. . (c) The cell viability of NRVMs treated with 100 μM H₂O₂ for 4 h detected by MTT assay. . (d) The representative images of flow cytometry using double staining of Annexin V and PI. (e–g) Statistical analysis of apoptotic, survival, and necrosis ratio of the flow cytometry data. ; vs. control; vs. +NC; vs. +AMO-155. +NC: H₂O₂+NC; +155: H₂O₂+miR-155; +AMO-155: H₂O₂+AMO-155; +155+AMO: H₂O₂+miR-155+AMO-155.

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