MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI

Figure 6

QKI as a target gene for miR-155 in cardiomyocytes. (a) The bioinformatic analysis showed that miR-155 had a binding site in the 3’-UTR of QKI mRNA of rat, human, and mouse species. (b) Relative luciferase activities of QKI wild-type (QKI-WT) UTRs and mutant UTRs of QKI (QKI-Mutant) were obtained by cotransfection of negative control miRNA or miR-155 mimics and the recombinant plasmid. Relative luciferase activity was calculated as the ratio of Renilla/firefly activities in the cells and normalized to those of the control; . vs. NC. (c) The protein levels of QKI-5 and QKI-6 in NRVMs were tested by western blot; . , vs. control; vs. miR-155; vs. AMO-155. (d) QKI mRNA level was detected by qRT-PCR; . vs. control; vs. +NC. (e) The protein expression of QKI-5 and QKI-6 in NRVMs were tested by western blot; . vs. control; , vs. +NC. (f) The protein expression of QKI-5 and QKI-6 in mice were tested by western blot; . vs. sham group; , vs. MI group.

(a)

(b)

(c)

(d)

(e)

(f)