Hypoxia-Induced ROS Contribute to Myoblast Pyroptosis during Obstructive Sleep Apnea via the NF-κB/HIF-1α Signaling Pathway

Figure 1

Increase in pyroptotic cell death in the gastrocnemius muscle of OSA mice. (a) TUNEL staining and (b) the percentage of TUNEL-positive nuclei in the gastrocnemius muscles of control and OSA mice. Scale μm. (c) Real-time PCR analysis of relative inflammatory gene expression in the gastrocnemius muscle of OSA mice (). (d–f) Caspase-1 (green) and NLRP3 (red) double staining in control and OSA mouse gastrocnemius sections. The staining results showed more nuclear colocalization of caspase-1 and NLRP3 (white arrow) in OSA mice. The white triangle indicates cytoplasmic colocalization. Scale  μm. The data are shown as the . , , ; NS: no significant difference.

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