Research Article
Hypoxia-Induced ROS Contribute to Myoblast Pyroptosis during Obstructive Sleep Apnea via the NF-κB/HIF-1α Signaling Pathway
Figure 3
Hypoxia activates caspase-1 in C2C12 cells. (a) Assay of caspase-1 activity in C2C12 cells with or without CoCl2 treatment (). (b) Immunofluorescence showed that caspase-1 clustered (arrow) at the nuclear membrane in CoCl2-treated cells, but not in control cells. Scale μm. (c, d) Western blot analysis of caspase-1 and cleaved caspase-1 p10 in C2C12 cells after CoCl2 treatment for 8 and 24 hours (). (e, f) Hoechst/PI double staining of C2C12 cells. A caspase-1 inhibitor (VX765) ameliorated CoCl2-induced cell death. Scale μm. The data are shown as the . , , ; NS: no significant difference.
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