Hypoxia-Induced ROS Contribute to Myoblast Pyroptosis during Obstructive Sleep Apnea via the NF-κB/HIF-1α Signaling Pathway

Figure 7

NAC downregulates HIF-1α expression activated by CoCl₂ in myoblasts. (a, b) Western blot analysis of HIF-1α in C2C12 cells at different time points after CoCl₂ treatment. (c) Immunofluorescence staining for HIF-1α in C2C12 myoblasts treated with or without CoCl₂ for 24 hours. CoCl₂ treatment induced HIF-1α nuclear expression. Scale  μm. (d–f) Western blot analysis of HIF-1α and phosphorylated NF-κB P65 in C2C12 cells after CoCl₂ treatment for 24 hours (). The data are shown as the . , , ; NS: no significant difference.

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